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Gas chromatographic determination of thymol

✍ Scribed by Matthew W. Noall; Vernon Knight; William W. Hargrove; Barry W. Elledge

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 345 KB
Volume: 69
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90559-x

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✦ Synopsis

Thymol in biological samples is analyzed by gas chromatography utilizing a 5% OV-25 column, a flame ionization detector, and eugenol as an internal standard. Samples are extracted with diethyl ether and analyzed without derivatization. The lower limit of detection of thymol in a sample is about 0.01 pg and quantitation is satisfactory in plasma at 0.05 pg/2 ml. Data was recorded digitally on magnetic tape and calculated off-line at a central facility. 10

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