Gas chromatographic determination of trace nitrite after derivatization with ethyl 3-oxobutanoate
โ Scribed by Takao Mitsuhashi
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 560 KB
- Volume
- 629
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
The reaction of 1-(2chloroethyl)-3-( t'rans-4-methylcyclohexyl)-1-nitrosourea (methyl-CCNU) with trifluoroacetic anhydride is reported. Gas-liquid chromatography of the resulting trifluoroacetyl derivative is applied to determine methyl-CCNU in plasma with a limit of detection of 1.5 pg ml-'.
2,4,5-T was extracted with acetone at below pH 1ร0 and the extract was concentrated. After adding 100 g litre~1 sodium chloride solution to the residual solution, 2,4,5-T was extracted with ethyl acetate ] hexane (20 ] 80 by volume). The extract was evaporated to dryness and the residue was dissolve