Gas chromatographic determination of quazepam and two major metabolites in human plasma

✍ Scribed by James M. Hilbert; James M. Ning; Gregory Murphy; Angel Jimenez; Nicola Zampaglione

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 417 KB
• Volume: 73
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600730421

✦ Synopsis

Two rapid, sensitive, and specific gas chromatographic (GC) methods for the quantitative determination of quazepam (I), 7-chloro-5-(2fluorophenyl-1,3-dihydro-1 -(2,2,2-trifluoroethyl)-2H -1,4-benzodiazepine-Zthione, and its major active plasma metabolites, the 2-0x0 compound (11) and the dealkylated 0x0 compound (111), have been developed; the first measures I and I1 and the second measures 111. The compounds are extracted from plasma with toluene and quantitated by electron-capture detection using the internal standard method. The methods are capable of quantitating plasma levels of I and I1 as low as 0.75 ng/mL and plasma levels of 111 as low as 1.5 ng/mL. Correlation coefficients of standard curves were >0.9995 for all compounds. Precision of the methods was measured at two different concentrations for each compound; the CV values were 3-6% for all three compounds. The recovery of all compounds was >80%, and the ratio of recovery of each cornpound to that of its internal standard did not vary at different concentrations, indicating appropriate internal standards have been selected for the methods. Quazepam metabolites, other benzodiazepines, and drugs which are potential comedication do not interfere with either method. The methods were shown to be suitable for investigating the bioavailability and pharmacokinetics of quazepam at therapeutic doses.

Keypbrases 0 Quazepam-gas chromatography two major metabolites, human plasma 0 Gas chromatography-quazepam and two major metabolites in human plasma 0 Metabolites-quazepam, human plasma, gas chromatography