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Further purification and characterisation of a new amylase found in barley

✍ Scribed by Alexander W. Macgregor; Jean Daussant; Marja-L Niku-Paavola

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
406 KB
Volume
30
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

An amylase, previously detected in barley and described as a new barley amylase, has been further purified by immuno‐affinity and ion exchange chromatography on CM‐cellulose Analysis by isoelectricfocusing and immunochemical techniques showed that thses enzyme preparation did not contain the normal α‐ and β‐amylases usually found barley and malt. The enzyme had a very low isoelectric point (ca pH 3.0) and was identified as an α‐amylase on the basis of its action pattern on amylose.

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