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Characterisation of feruloyl esterase activity in barley

✍ Scribed by Sancho, Ana I; Faulds, Craig B; Bartolom�, Bego�a; Williamson, Gary

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
106 KB
Volume
79
Category
Article
ISSN
0022-5142

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✦ Synopsis

Feruloyl esterase activity has been detected in barley grain and germinated barley. No differences have been found between the values of activity against methyl ferulate in barley crude extracts (1.05 » 0.10 mU mg-1 protein) with and without sterilisation (P > 0.05), indicating that the activity is native to the barley grain rather than due to microbial contamination. The highest value of esterase activity was detected after 2 day growth, although an overall downward trend was observed over a 6 day germination period at 25ÄC. The and of the crude barley esterase/s on methyl K m V max ferulate were 0.34 » 0.05 mM and 0.76 » 0.03 mU mg-1 protein, respectively. The crude extracts were also active upon the methyl esters of sinapic, p-coumaric and caþ eic acids, and upon a feruloylated arabino-oligosaccharide but not upon the methyl ester of vanillic acid. (Ara 2 F), 1999 Society of Chemical Industry

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