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Functional characterization of the penicillin biosynthetic gene cluster of Penicillium chrysogenum Wisconsin54-1255

✍ Scribed by Marco A. van den Berg; Ilja Westerlaken; Chris Leeflang; Richard Kerkman; Roel A.L. Bovenberg


Book ID
116481851
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
519 KB
Volume
44
Category
Article
ISSN
1087-1845

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The low penicillin-producing, single gene copy strain Wis54-1255 was used to study the effect of overexpressing the penicillin biosynthetic genes in Penicillium chrysogenum. Transformants of Wis54-1255 were obtained with the amdS expression-cassette using the four combinations: pcbAB, pcbC, pcbC-pen