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Cloning and Heterologous Expression of the Penicillin Biosynthetic Gene Cluster from Penicillium chrysogenum

✍ Scribed by Smith, David J. ;Burnham, Martin K. R. ;Edwards, Jeffrey ;Earl, Alison J. ;Turner, Geoffrey

Book ID
109901191
Publisher
Nature Publishing Group
Year
1990
Tongue
English
Weight
732 KB
Volume
8
Category
Article
ISSN
1087-0156

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Quantitative analysis of Penicillium chrysogenum Wis54-1255 transformants overexpressing the penicillin biosynthetic genes
✍ Hanne Aae Theilgaard; Marco A. van den Berg; CΓ©line A. Mulder; Roel A.L. Bovenbe πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 202 KB

The low penicillin-producing, single gene copy strain Wis54-1255 was used to study the effect of overexpressing the penicillin biosynthetic genes in Penicillium chrysogenum. Transformants of Wis54-1255 were obtained with the amdS expression-cassette using the four combinations: pcbAB, pcbC, pcbC-pen