𝔖 Bobbio Scriptorium
✦   LIBER   ✦

FROM HORMONE SIGNAL, VIA THE CYTOSKELETON, TO CELL GROWTH IN SINGLE CELLS OF TOBACCO

✍ Scribed by Kris Vissenberg; Anne-Helene Quelo; Kristin Van Gestel; Geert Olyslaegers; Jean-Pierre Verbelen

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
819 KB
Volume
24
Category
Article
ISSN
1065-6995

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Cultured mesophyll protoplasts of Nicotiana tabacum L. can be hormonally induced into different developmental pathways. In a medium containing auxins (NAA) and cytokinins (BAP) cells divide and eventually give rise to calli. When only auxins are present cells elongate and finally differentiate into very long tubular cells. We focused on the sequence of events leading to elongation. When cultured in a high (1mg/l) auxin concentration elongating cells seem to pass a certain threshold and increase their nuclear DNA up to about 16C. Cells cultured in a low (0.065mg/l) auxin concentration only have C‐values up to 4C, are unable to pass this threshold and finally fail to elongate. Besides the concentration dependence of the auxin signal, the efflux of auxin seems to be necessary for elongation since addition of TIBA drastically reduces the amount of elongating cells. Concomitant with the changes in nuclear physiology, auxin‐induced axiality is seen as sequential rearrangements of microtubules and actin‐filaments and of cell wall cellulose microfibrils from β€˜randomly’ arranged in spherical cells to an orientation perpendicular to the long axis of elongating cells.

πŸ“œ SIMILAR VOLUMES

Growth energetics in the production of b
Growth energetics in the production of bacterial single cell protein from Methanol
✍ J. W. Drozd; S. J. Wren πŸ“‚ Article πŸ“… 1980 πŸ› John Wiley and Sons 🌐 English βš– 464 KB

## Abstract Bacteria grown on methanol exhibit a poor efficiency of energy conservation, which is mainly due to the low P/O ratio of 1 associated with methanol oxidation. Thermodynamic considerations indicate that a P/O ratio of at least 2 is possible for this step in substrate oxidation. This low

Properties of growth hormone receptors i
Properties of growth hormone receptors in relation to the adipose conversion of 3T3 cells
✍ Tracy Nixon; Howard Green πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 624 KB

## Abstract Cultured preadipose 3T3 cells undergo a process of differentiation in which they convert to adipose cells. Growth hormone promotes this conversion. Since 3T3 sublines vary in their susceptibility to adipose conversion, it was of interest to examine the properties of the growth hormone r

Involvement of Shc in the signaling resp
Involvement of Shc in the signaling response of human prostate tumor cell lines to epidermal growth factor
✍ John Gresham; Patricia Margiotta; Ann J. Palad; Kenneth D. Somers; Peter F. Blac πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 French βš– 134 KB πŸ‘ 2 views

Autocrine growth factors for the epidermal growth factor receptor (EGFR) have been identified in prostate tumors, implicating a role for EGFR in the progression of prostate cancer. To investigate early signaling mechanisms used by the EGFR in prostate tumor cells, we have characterized the involveme