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Forward and Reverse DNA Sequencing in a Single Reaction

✍ Scribed by Dirk van den Boom; Christian Jurinke; Andreas Ruppert; Hubert Köster

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
719 KB
Volume
256
Category
Article
ISSN
0003-2697

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## Abstract **One's enough**: The direct observation of a DNA‐polymerase‐based “sequencing engine” using single‐molecule detection recently allowed single‐molecule sequencing by synthesis in real time. Nucleotides with a fluorescent marker at the 5′‐phosphate unit and zero‐mode waveguides are cruci