𝔖 Bobbio Scriptorium
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Formation of α-keto acids from amino acids using immobilized bacteria and algae

✍ Scribed by P. Wikström; E. Szwajcer; P. Brodelius; K. Nilsson; K. Mosbach

Publisher
Springer Netherlands
Year
1982
Tongue
English
Weight
358 KB
Volume
4
Category
Article
ISSN
0141-5492

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✦ Synopsis

Both free and immobilized cells of the algae Chlorella vulgaris and Anacystis nidulans contain aminoacid oxidase (AAO) activity which is increased by illumination with red light. Both immobilized species are photosynthetically active. By co-immobilizing Chlorella with bacterial cells (Providencia sp. PCM 1298) containing high AA0 activity an increased production of keto acid (up to tenfold) is observed due to improved oxygen supply.

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## Abstract Diazotization of α‐amino acids in 48:52 (__w__/__w__) hydrogen fluoride/pyridine along with excess of potassium halide results in the corresponding α‐halocarboxylic acids in good to excellent yields __(Table 1__ and __2)__.