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Formation of fibroblast colonies in monolayer cultures of the spleen of irradiated and normal mice

โœ Scribed by A. Ya. Fridenshtein; G. N. Kuz'menko

Publisher
Springer US
Year
1971
Tongue
English
Weight
616 KB
Volume
71
Category
Article
ISSN
0007-4888

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Fibroblast colonies in monolayer culture
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## Abstract In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 ร— 10~5~ cells in the culture. Normal human bone marr

Factors influencing hematopoietic spleen
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A variety of erythropoietic stimuli influenced the number of endogenous spleen colonies in irradiated mice and the number of transplantable colony forming cells in the spleen and marrow of unirradiated mice. Bleeding was the most effective stimulus. Bleeding before irradiation resulted in a 30-fold