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Clone formation in monolayer cultures of bone marrow and spleen

โœ Scribed by R. K. Chailakhyan; A. Ya. Fridenshtein; A. V. Vasil'ev

Publisher
Springer US
Year
1970
Tongue
English
Weight
387 KB
Volume
69
Category
Article
ISSN
0007-4888

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## Abstract In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 ร— 10~5~ cells in the culture. Normal human bone marr