## Abstract Two separate binding proteins, one specific for folate and the other for thiamine, have been isolated from membrane fragments of Lactobacillus casei. Purification to homogeneity was achieved by fractionation of the Tritonβsolubilized proteins with microgranular silica (Quso Gβ32) and Se
Folate levels and methylation of CDKI proteins
β Scribed by Gabriele Buda; Valentina Maggini; Sara Galimberti; Roberto Barale; Anna Maria Rossi; Mario Petrini
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 93 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0145-2126
No coin nor oath required. For personal study only.
β¦ Synopsis
The purpose of this study was to investigate WT1 expression levels in childhood acute leukemia. Bone marrow from 14 children with acute leukemia at diagnosis and from 7 children with solid tumors without bone marrow involvement (control group) was studied. Five of the 14 patients (35.7%), expressed high levels of WT1. Four of the five WT1 positive patients with additional adverse prognostic factors, have succumbed to their disease. The results of this study are in accordance with the fact that high levels of WT1 expression have been reported in patients with an unfavorable outcome.
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A simple, rapid, and quantitative procedure for the protection of reduced S-S bonds in proteins by selective methylation with dimethylsuifate is described. The formed S-methylcysteine is a stable derivative well suited for ion-exchange chromatographic (iec) or gas-liquid chromatographic (glc) analys
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