✦ LIBER ✦

S-methylation of cysteine residues in peptides and proteins with dimethylsulfate

✍ Scribed by Julius Eyem; Jörgen Sjödahl; John Sjöquist

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 595 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90217-7

No coin nor oath required. For personal study only.

✦ Synopsis

A simple, rapid, and quantitative procedure for the protection of reduced S-S bonds in proteins by selective methylation with dimethylsuifate is described. The formed S-methylcysteine is a stable derivative well suited for ion-exchange chromatographic (iec) or gas-liquid chromatographic (glc) analysis. Its methylor phenyl-t~obydantoin is easily analyzed by gas-liquid chromatography after t~methy~silylation or by thin-layer chromatography (tic).

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