FMOC solid phase synthesis of an endothelin converting enzyme substrate
โ Scribed by HANDA, BALRAJ K. ;KEECH, ELIZABETH
- Book ID
- 115099296
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 398 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0367-8377
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๐ SIMILAR VOLUMES
Endothelin-converting enzyme-1 (ECE-1) is a membrane-bound zinc-metallopeptidase that is related to neprilysin in amino acid sequence. A major in vivo function of ECE-1 is the proteolytic conversion of big endothelin-1 to endothelin-1, one of the most potent vasconstricting peptides known. Although
The incorpmation of O-phosphotyrosine into synthetic peptides using Pmoc-Tyr(PO Me of the modef & )-OH in the Fmoc-polyamide procedure is described uith the preparation yr-tridecapeptide sequence H-Arg-Leu-IZe-GZu-Asp-Asn-GZu-flyr-Thr-Ala-Arg-GZn-GZy-OH.
To synthesize peptide thioesters directly on a solid support for use as substrate analogues for thioesterases in non-ribosomal peptide synthases, we modified a reagent compatible with Fmoc solid-phase peptide synthesis that efficiently removes the protecting group while keeping the thioester intact.