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Fluorometric assay of tubulin-colchicine complex

✍ Scribed by Takao Arai; Tsuneo Okuyama

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 431 KB
Volume: 69
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90146-3

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✦ Synopsis

A fluorometric assay method for the tubulin-colchicine complex has been developed. This assay method is based on the fact that the binding of colchicine to tubulin leads to a 300-fold enhancement of fluorescence intensity of colchicine at 430 nm. Since the excitation wavelength can be set at 350 nm, far from an absorption band of tubulin, the assay does not necessitate the separation of the tubulin-colchicine complex from free colchicine. Fluorescence intensity is linear up to 2 mg/ml of the tubulin-colchicine complex.

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