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A new colchicine binding assay for tubulin

✍ Scribed by Peter Sherline; Cynthia K. Bodwin; David M. Kipnis

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
415 KB
Volume
62
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple and sensitive procedure is described for assaying tubulin. the subunit protein of microtubules. Tubulin is equilibrated with ['Hlcolchicine after which the remaining free colchicine is removed by adsorption to activated charcoal. Values for the amount of tubulin in various rat tissues have been determined by both the charcoal method and by gel filtration on Sephadex G-100, and in all cases the values determined by the two methods correspond closely.

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