A sensitive competency assay for concanavalin A binding

✍ Scribed by Paul M. Horowitz; Philip G. Phillips

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 207 KB
• Volume: 74
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90321-3

✦ Synopsis

A standardized assay method has been developed for assessing the binding capacity of derivitized concanavalin A (con A). The method takes advantage of the fact that concanavalin A binds to Sephadex G-75 in an cr-methyt-rr-mannopyranoside-inhibitable manner. Using this binding assay we have found that cx-methyl-D-mannopyranoside inhibition of ['251]concanavalin A to Sephadex is greater than 95%. In addition, this sugar is able to remove more than 99% of ['251]concanavalin A previously bound to Sephadex. Competition studies will allow the use of this procedure to characterize any con A sample under the exact conditions used for cell-binding studies.