The use o f 6-(N-acetyl-~-phenylalanyl)-aminoluciferin as a novel substrate for achymotrypsin has been demonstrated. The kinetic parameters determined are K M = 0.38 mmol/L, kcat = 6.5 s-' and kcat/kM = 17,100 (L/mol s). The test principle o f the coupled assay is the release o f aminoluciferin by e
Fluorometric assay of α-chymotrypsin
✍ Scribed by Benon H.J. Bielski; Simon Freed
- Publisher
- Elsevier Science
- Year
- 1964
- Tongue
- English
- Weight
- 323 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0003-2697
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