๐”– Bobbio Scriptorium
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Fluorometric assay for urinary indapamide

โœ Scribed by Peter E. Grebow; Jo A. Treitman; Anne K. Yeung

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
424 KB
Volume
67
Category
Article
ISSN
0022-3549

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โœฆ Synopsis

A sensitive fluorescence method for the determination of indapamide was developed. Reaction of indapamide with sodium hydroxide at 100 degrees yielded a fluorescent product, and addition of formaldehyde to the fluorescent product increased its fluorescence intensity by a factor of three. The assay is sensitive to levels of indapamide of 0.025 microgram/ml in an aqueous solution, and a linear response between 0.025 and 2.0 microgram/ml was observed. The procedure was adapted to the analysis of intact indapamide in urine. Concentrations of indapamide of 0.05 microgram/ml can be detected in dogs given 20 mg of the drug.

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