Fluorometric assay for 1-deoxyfructose

✍ Scribed by William L. Dills Jr.; Geraldine M. McDonough

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 496 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90593-x

✦ Synopsis

A sensitive fluorometric assay has been developed for the measurement of I-deoxyfructose in biological fluids. Samples containing I-deoxyfructose are incubated with an equal volume of 0.01 M 3,5-diaminobenzoic acid in 5.0 M phosphoric acid in a boiling-water bath for 15 min. The fluorescent product has an emission maximum at 502 nm and an excitation maximum at 3% nm. Fluorescence is proportional to I-deoxyfructose concentrations over a range from 0.002 to 1 .O mM. The method can be used to detect as little as 0.03 pmol of I-deoxyfructose in deproteinized blood and in urine and no interference is observed with glucose, fructose, pyruvate, or ketone bodies. The method will also detect I-deoxytagatose, 2-deoxyaldohexoses, and -pentoses, 2,5-anhydromannose, and a number of 2-, 3-, 4, and 5-mono-or bis(hydroxymethyl)furans. The fluorescence properties of the products formed from all of the above compounds are similar suggesting structural similarities of the adducts formed and possible mechanistic similarities of the reactions involved.

' All sugars mentioned in this manuscript are of the D-configuration.

2 Abbreviation used: DABA, 2,5-diaminobenzoic acid.