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Fluorometric analysis of DNA in cell cultures

✍ Scribed by K. Janakidevi; C.D. Murray; C. Sell; P. Held

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 267 KB
Volume: 172
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90413-7

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✦ Synopsis

A simple, reproducible fluorescent assay has been described which can be used confidently at microgram and submicrogram levels. The method is most suitable for cell cycle and cell growth analysis using [3H]thymidine as the radioactive tracer. A modified method is also described for preparing diaminobenzoic acid hydrochloride.

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