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Fluorimetric detection of serum corticosterone using high-performance liquid chromatography

โœ Scribed by Steven R. Mason; Leigh C. Ward; Paul E.B. Reilly

Publisher
Elsevier Science
Year
1992
Weight
468 KB
Volume
581
Category
Article
ISSN
0378-4347

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A new method for the simultaneous determination of cortisol and cortisone in serum by high-performance liquid chromatography with fluorimetric detection has been developed. The two steroids were derivatized by treatment with 9-anthroyl nitrile in triethylamine-acetonitrile to give the fluorescent es