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Assay for dopamine β-hydroxylase in human plasma and rat serum by high-performance liquid chromatography with fluorimetric detection

✍ Scribed by Myung-Koo Lee; Hitoshi Nohta; Yosuke Ohkura


Publisher
Elsevier Science
Year
1987
Weight
534 KB
Volume
421
Category
Article
ISSN
0378-4347

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Fluorescence assay for dopamine β-hydrox
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As a model for the assay of enzyme activity by high-performance liquid chromatography, a simple, sensitive and specific fluorescence assay for dopamine P-hydroxylase (DBH) activity was devised using o-phthalaldehyde as reagent. Tyramine was used as a substrate and was incubated under optimal conditi