Fluorescence assay for dopamine β-hydrox
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Keisuke Fujita; Toshiharu Nagatsu; Kazuhiro Maruta; Ryoji Teradaira; Hidehiro Be
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Article
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1977
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Elsevier Science
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English
⚖ 596 KB
As a model for the assay of enzyme activity by high-performance liquid chromatography, a simple, sensitive and specific fluorescence assay for dopamine P-hydroxylase (DBH) activity was devised using o-phthalaldehyde as reagent. Tyramine was used as a substrate and was incubated under optimal conditi