The simultaneous determination of serum cortisol and cortisone by high-performance liquid chromatography with fluorimetric detection

✍ Scribed by Junichi Goto; Fazel Shamsa; Nobuharu Goto; Toshio Nambara

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 446 KB
• Volume: 1
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(83)80011-9

✦ Synopsis

A new method for the simultaneous determination of cortisol and cortisone in serum by high-performance liquid chromatography with fluorimetric detection has been developed. The two steroids were derivatized by treatment with 9-anthroyl nitrile in triethylamine-acetonitrile to give the fluorescent esters through the 21-hydroxyl group. These derivatives were efficiently separated on a straight-phase Cosmosil 5SL column with hexane-ethyl acetate (65) as eluent. The detection limit was 20 fmol, using the 9anthroyl derivative of prednisolone as an internal standard. The use of Sep-Pak Cl8 and Clin-Elut cartridges proved to be efficient for the clean-up of cortisol and cortisone in serum samples.