The simultaneous determination of serum
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Junichi Goto; Fazel Shamsa; Nobuharu Goto; Toshio Nambara
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Article
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1983
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Elsevier Science
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English
โ 446 KB
A new method for the simultaneous determination of cortisol and cortisone in serum by high-performance liquid chromatography with fluorimetric detection has been developed. The two steroids were derivatized by treatment with 9-anthroyl nitrile in triethylamine-acetonitrile to give the fluorescent es