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Fluorescently labeled model DNA sequences for exonucleolytic sequencing

✍ Scribed by Zeno Földes-Papp; Bernhard Angerer; Per Thyberg; Michael Hinz; Stefan Wennmalm; Waltraud Ankenbauer; Hartmut Seliger; Arne Holmgren; Rudolf Rigler

Book ID
119523957
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
228 KB
Volume
86
Category
Article
ISSN
0168-1656

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A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of