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Automated sequencing of fluorescently labelled DNA by chemical degradation

✍ Scribed by Rosenthal, Andre ;Sproat, Brian ;Voss, Hartmut ;Stegemann, Josef ;Erfle, Holger ;Zimmermann, Jürgen ;Coutelle, Charles ;Ansorge, Wilhelm

Book ID
117992625
Publisher
Informa plc
Year
1990
Tongue
English
Weight
346 KB
Volume
1
Category
Article
ISSN
1042-5179

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A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of