Identification and characterization of the cellular proteins that specifically bind to the immunosupprassive drugs, cyclosporine (CsA), FKS06, and rapamycin is necessary to understand their mechanism of action. We have isolated and partially characterized a 52 kDa binding protein (BP) from calf thym
Fluorescent Probes to Characterise FK506-Binding Proteins
✍ Scribed by Christian Kozany; Andreas März; Christoph Kress; Felix Hausch
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 438 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4227
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Cytosolic proteins that specifically bind cyclosporine A and FK-506 were isolated and purified from the JURKAT human T-helper cell line. These binding proteins were purified by affinity, molecular weight exclusion and weak cation exchange column chromatography. Radiolabeled cyclosporine A specifical