✦ LIBER ✦

Purification and characterization of cyclosporine and FK-506 binding proteins from a human T-helper cell line

✍ Scribed by Edmund W. Palaszynski; James G. Donnelly; Steven J. Soldin

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 836 KB
Volume: 24
Category: Article
ISSN: 0009-9120
DOI: 10.1016/0009-9120(91)90252-a

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✦ Synopsis

Cytosolic proteins that specifically bind cyclosporine A and FK-506 were isolated and purified from the JURKAT human T-helper cell line. These binding proteins were purified by affinity, molecular weight exclusion and weak cation exchange column chromatography. Radiolabeled cyclosporine A specifically bound to a -17 kDa molecule which is cyclophilin and also bound to a -50 kDa protein(s). Radiolabeled FK-506 did not bind to the -17 kDa molecular weight protein, but specifically bound to soluble -10 kDa and -50 kDa proteins.