𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry

✍ Scribed by S. K. Doberstein; G. Wiegand; L. M. Machesky; T. D. Pollard

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
405 KB
Volume
20
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

✦ Synopsis

We report here a quick and inexpensive method for preparing standards of known fluorochrome content for calibration and quantitation of flow cytometry fluorescence signals. Erythrocyte ghosts prepared b y hypotonic lysis are U e d with solutions containing fluorescently labeled dextran. Standards prepared by this technique have a MITOW range of fluorescence and a linear response of fluorescence to fluorochrome content up to 2 x lo6 fluorochrome molecules/cell. The volume of ghost standard particles is roughly 70 femtoliters (fl)/cell. The fluorescence of ghost standards is nearly identical to that of commercially available microbead standards of similar fluorochrome content. Ghost standards have stable fluorescence for at least 3 weeks at 4OC. These standards can be made with any fluorochrome or combination of fluorochromes over a wide concentration range.

πŸ“œ SIMILAR VOLUMES

Standardizing flow cytometry: A classifi
Standardizing flow cytometry: A classification system of fluorescence standards used for flow cytometry
✍ A. Schwartz; G. E. Marti; R. Poon; J. W. Gratama; E. FernΓ‘ndez-Repollet πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 69 KB πŸ‘ 2 views

The growing number of standards commercially available in the field of flow cytometry makes it difficult to know which standards to use to obtain a desired level of quality assurance. A classification system of fluorescence standards has been developed on the basis of their physical characteristics.

Terminology and nomenclature for standar
Terminology and nomenclature for standardization in quantitative fluorescence cytometry
✍ L. Omar Henderson; Gerald E. Marti; Adolfas Gaigalas; W. Harry Hannon; Robert F. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 86 KB

Terminology in any field is a complex mix of established conventions, accepted usages, disputed terms, and occasional misnomers. The terminology that has evolved for quantitative fluorescence cytometry (QFCM) is especially multifarious, in part because QFCM encompasses a range from subjective visual

Performance of calibration standards for
Performance of calibration standards for antigen quantitation with flow cytometry
✍ R. Lenkei; J. W. Gratama; G. Rothe; G. Schmitz; J. L. D'hautcourt; A. Γ…rekrans; πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 81 KB πŸ‘ 2 views

In the frame of the activities initiated by the Task Force for Antigen Quantitation of the European Working Group on Clinical Cell Analysis (EWGCCA), an experiment was conducted to evaluate microbead standards used for quantitative flow cytometry (QFCM). An unified window of analysis (UWA) was estab