𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Terminology and nomenclature for standardization in quantitative fluorescence cytometry

✍ Scribed by L. Omar Henderson; Gerald E. Marti; Adolfas Gaigalas; W. Harry Hannon; Robert F. Vogt Jr.

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
86 KB
Volume
33
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

✦ Synopsis

Terminology in any field is a complex mix of established conventions, accepted usages, disputed terms, and occasional misnomers. The terminology that has evolved for quantitative fluorescence cytometry (QFCM) is especially multifarious, in part because QFCM encompasses a range from subjective visual assessments to objective photon counts. Thus, while descriptive terms such as ''dim'' and ''bright'' are still quite useful, quantitative terms such as ''binding capacity'' should be used with collective understanding of their exact meanings. This article reviews current usage and proposes definitions that, with refinement from suppliers and users of QFCM technology, can provide the required clarity. Cy-

πŸ“œ SIMILAR VOLUMES

Fluorescent erythrocyte ghosts as standa
Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry
✍ S. K. Doberstein; G. Wiegand; L. M. Machesky; T. D. Pollard πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 405 KB πŸ‘ 1 views

We report here a quick and inexpensive method for preparing standards of known fluorochrome content for calibration and quantitation of flow cytometry fluorescence signals. Erythrocyte ghosts prepared b y hypotonic lysis are U e d with solutions containing fluorescently labeled dextran. Standards pr

Quantitative fluorescence flow cytometry
Quantitative fluorescence flow cytometry: A comparison of the three techniques for direct and indirect immunofluorescence
✍ Stefan Serke; Antje van Lessen; Dieter Huhn πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 222 KB πŸ‘ 2 views

Three types of microbead calibrators available for quantitative fluorescence flow cytometry have been studied in parallel using a variety of monoclonal antibodies (MoAbs). The QIFI kit is designed for indirect immunofluorescence (IF), and both the Quantum Simply Cellular (QSC) assay and the Quanti-B