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Fluorescence Studies of the Nicotinic Acetylcholine Receptor in its Membrane Environment

✍ Scribed by Francisco J. Barrantes; Silvia Antollini; Ramiro Massol


Book ID
110393845
Publisher
Springer
Year
1999
Tongue
English
Weight
842 KB
Volume
19
Category
Article
ISSN
0144-8463

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An apparatus has been developed that allows photoaffinity ligands to be crossed-linked to milligram quantities of membrane proteins with maximum attainable yield following contact times of approximately 1 ms. The apparatus consisted of three parts: a conventional rapid mixing unit, a novel freeze-qu