Fluorescence Studies of the Acetylcholine Receptor: Structure and Dynamics in Membranes and Cells
β Scribed by Francisco J. Barrantes
- Book ID
- 110325578
- Publisher
- Springer
- Year
- 2001
- Tongue
- English
- Weight
- 154 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1053-0509
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Receptor binding studies with the conventional method require the use of radioactively labelled ligands and include several washing steps. In certain cases, the halflife of the receptor-ligand complex is often shorter or similar to the time required for separation. Interactions between certain ligan
Acetylcholine receptor (AcChR) enriched membrane fragments from Torpedo californica electroplax were labeled by in situ photogenerated nitrenes from a hydrophobic fluorescent probe, pyrene-1-sulfonyl azide. Preferential photolabeling of membrane proteins, mainly AcChR, has been achieved and there is