Fluorescence Spectroscopy: New Methods and Applications

✍ Scribed by Steven G. Schulman (auth.), Professor Dr. Otto S. Wolfbeis (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1993
• Tongue: English
• Leaves: 315
• Edition: 1
• Category: Library

✦ Synopsis

"Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC)
The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.

✦ Table of Contents

Front Matter....Pages I-XX
Front Matter....Pages 1-1
Fluorescence Spectroscopy: Where We Are and Where We’re Going....Pages 3-12
Interactions and Kinetics of Single Molecules as Observed by Fluorescence Correlation Spectroscopy....Pages 13-24
Fast Optical Imaging Techniques....Pages 25-30
Kinetic Studies on Fluorescence Probes Using Synchrotron Radiation....Pages 31-47
Dynamics and Geometry in Dimeric Flavoproteins from Fluorescence Relaxation Spectroscopy....Pages 49-63
Fluorescence Spectroscopy on Light Scattering Materials....Pages 65-78
Optical Sensors Based on Fluorescence Quenching....Pages 79-89
Front Matter....Pages 91-91
Fluorescence in Forest Decline Studies....Pages 93-109
Fluorescence Microscopy Studies of Structure Formation in Surfactant Monolayers....Pages 111-127
Fluorescence Lifetime Imaging and Application to Ca 2+ Imaging....Pages 129-147
Ether Phospholipids in Membranes: Applications of Phase and Steady-State Fluorometry....Pages 149-157
Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models....Pages 159-171
Optical Detection of Intracellular Ion Concentrations....Pages 173-185
Front Matter....Pages 187-187
Analytical Applications of Very Near-IR Fluorimetry....Pages 189-196
Fluorescence Detection in Flow Injection Analysis....Pages 197-212
Fluorescence Spectroscopy in Environmental and Hydrological Sciences....Pages 213-241
Front Matter....Pages 243-243
Fluorescence Polarisation Immunoassay....Pages 245-258
Progress in Delayed Fluorescence Immunoassay....Pages 259-266
Chemiluminescence Detection in Immunochemical Techniques. Applications to Environmental Monitoring....Pages 267-278
Front Matter....Pages 279-279
Fluorescence Transients in Neurobiology: Applications of Voltage Sensitive and Ion Indicator Dyes....Pages 281-290
Front Matter....Pages 279-279
Optical Monitoring of Postsynaptic Potential in the Early Embryonic Avian Brain Stem Using a Voltage-Sensitive Dye....Pages 291-303
Back Matter....Pages 305-312

✦ Subjects

Analytical Chemistry; Physical Chemistry; Biochemistry, general; Human Physiology