Fluorescence Spectroscopy and Microscopy: Methods and Protocols

✍ Scribed by Adam Matthew Gilmore (auth.), Yves Engelborghs, Antonie J.W.G. Visser (eds.)

• Publisher: Humana Press
• Year: 2014
• Tongue: English
• Leaves: 812
• Series: Methods in Molecular Biology 1076
• Edition: 1
• Category: Library

✦ Synopsis

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

✦ Table of Contents

Front Matter....Pages i-xxi
Front Matter....Pages 1-1
Front Matter....Pages 3-27
Front Matter....Pages 29-42
Front Matter....Pages 43-51
Front Matter....Pages 53-74
Front Matter....Pages 75-75
Front Matter....Pages 77-95
Front Matter....Pages 97-112
Front Matter....Pages 113-169
Back Matter....Pages 171-214
....Pages 215-256

✦ Subjects

Imaging / Radiology; Biological Microscopy; Spectroscopy/Spectrometry