Fluorescence quenching by acrylamide in micellar solutions

✍ Scribed by M.A. Rubio; E.A. Lissi

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 435 KB
• Volume: 71
• Category: Article
• ISSN: 1010-6030
• DOI: 10.1016/1010-6030(93)85070-o

✦ Synopsis

Quenching of the fluorescence of methylpyrene and anthracene derivatives by acrylamide in micelles follows a linear Stern-Volmer relationship. Incorporation of the fluorophores inside the micelles leads to a decrease in the quenching rates, and the bimolecular rate constants, obtained in terms of the analytical acrylamide concentration, are considerably smaller in cetyltrimethylammonium chloride (CTAC) and poly(oxyethylene)dodecyl ether (Brij 35) micelles than in sodium dodecylsulphate (SDS) micelles. By comparison of these data with those obtained using indole derivatives, it is concluded that the strong protection observed, particularly in CTAC micelles, is due to a reduction in the intramicellar bimolecular quenching rate constant. Increasing the SDS concentration or the addition of n-hexanol or NaCl leads to a reduction in the interaction rate due to partial dehydration of the micellar interface.