๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Acrylamide quenching of fluorescence in micellar environments

โœ Scribed by Christopher P. Loran; Ray von Wandruszka

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
378 KB
Volume
258
Category
Article
ISSN
0003-2670

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โœฆ Synopsis

AbStIXt Acrylanude 1s a hydrophdx fluorescence quencher that nonetheless readily enters the hydmpholnc mterlor of detergent nucelles m aqueous solution No such movement IS observed between bulk aqueous and orgamc phases &molecular quenchmg constants m both normal and reverse nucelles mchcate that quenchmg of nucelhzed pyrene fluorescence by acrylanude IS a dtisional process Considerations of energy transfer from donor nacelles to a solubdlzed pyrene acceptor, II/I, ratios of the probe, and comparison with quenchmg by bronude Ions suggest that the hydrocarbon extrenuty of acrylamlde has the alxhty to penetrate across the polarity boundaxy of normal rmcelles and quench the fluorescence of a probe located Just mslde It &ywords Acrylarmde quenclung, Fluorescence, MIcelIes, Quenching

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