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Fluorescence measurements of nucleotide association with the Na+/K+-ATPase

โœ Scribed by Promod R. Pratap; Lydia O. Mikhaylyants; Natalie Olden-Stahl


Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
624 KB
Volume
1794
Category
Article
ISSN
1570-9639

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โœ Ellen L.P. Chan; R. Swaminathan ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 369 KB

A rapid and automated assay for inhibitors of Na+, K(+)-ATPase was developed by determining the amount of inorganic phosphorus (Pi) released by Na+,K(+)-ATPase in a centrifugal analyzer. This method avoids long incubation, strong acids and centrifugation as in the conventional manual method. The coe