✦ LIBER ✦

A rapid assay for the measurement of Na+,K+-ATPase inhibitors

✍ Scribed by Ellen L.P. Chan; R. Swaminathan

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 369 KB
Volume: 25
Category: Article
ISSN: 0009-9120
DOI: 10.1016/0009-9120(92)80040-n

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✦ Synopsis

A rapid and automated assay for inhibitors of Na+, K(+)-ATPase was developed by determining the amount of inorganic phosphorus (Pi) released by Na+,K(+)-ATPase in a centrifugal analyzer. This method avoids long incubation, strong acids and centrifugation as in the conventional manual method. The coefficients of variation of intra- and inter-assay at ouabain concentration 0.5 mumol/L were 1.0 and 1.4%, respectively. The method is quick, reproducible and easy compared with current techniques.

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