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Toward an understanding of the fluorescence intensity changes observed on fluorescein 5′-Isothiocyanate-Na+,K+-ATPase

✍ Scribed by E. Grell; E. Lewitzki; H. Ruf; K. Brand; F. W. Schneider; Th. von der Haar; K. A. Zachariasse

Publisher
Springer
Year
1994
Tongue
English
Weight
272 KB
Volume
4
Category
Article
ISSN
1053-0509

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✍ Shwu-Hwa Lin; Larry D. Faller 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 114 KB

Specific labeling is required for energy transfer measurements and to avoid artifacts in the use of fluorophores as reporter groups. Therefore, a method for specific modification by one of the most popular reagents for P-type ATPases (fluorescein 5-isothiocyanate) has been developed. Sulfhydryl reag