Fluorescence correlation spectroscopy (FCS) is a fluorescence microscopy technique that allows the study of molecular interactions in extremely low volumes, at nanomolar concentrations, even when binding is not accompanied by a fluorescence change. It can be applied directly in living cells. FCS cle
β¦ LIBER β¦
Fluorescence-based analysis of enzymes at the single-molecule level
β Scribed by Kerstin Blank; Gert De Cremer; Johan Hofkens
- Book ID
- 112011806
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 446 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1860-6768
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## Abstract The TAR element of HIV and the viral protein Tat form a molecular switch regulating transcriptional efficiency in HIV. We show that fluorescence correlation spectroscopy at the single molecule level is a powerful method to study the association between a Tatβderived peptide and TAR frag