Providing researchers with insights into the complex world of cellular signalling, protein interactions are an increasingly important aspect of live cell research. Advanced developments in fluorescence proteins have enabled live cell microscopy to become an important tool in these studies. Fluoresce
Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) at the Single Molecule Level
β Scribed by Philip Tinnefeld; Volker Buschmann; Dirk-Peter Herten; Kyung-Tae Han; Markus Sauer
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 953 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1438-5163
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Fluorescence correlation spectroscopy (FCS) is a fluorescence microscopy technique that allows the study of molecular interactions in extremely low volumes, at nanomolar concentrations, even when binding is not accompanied by a fluorescence change. It can be applied directly in living cells. FCS cle
## Abstract The TAR element of HIV and the viral protein Tat form a molecular switch regulating transcriptional efficiency in HIV. We show that fluorescence correlation spectroscopy at the single molecule level is a powerful method to study the association between a Tatβderived peptide and TAR frag