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Fluorescence correlation spectroscopy: molecular recognition at the single molecule level

✍ Scribed by E. Van Craenenbroeck; Y. Engelborghs

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
115 KB
Volume
13
Category
Article
ISSN
0952-3499

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✦ Synopsis

Fluorescence correlation spectroscopy (FCS) is a fluorescence microscopy technique that allows the study of molecular interactions in extremely low volumes, at nanomolar concentrations, even when binding is not accompanied by a fluorescence change. It can be applied directly in living cells. FCS clearly considerably extends the possibilities of the classical techniques used in molecular recognition studies and can be considered to belong to a growing group of techniques that allow detection at the single molecule level. In this review, several applications of FCS, both in vitro and in vivo, will be discussed.

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## Abstract The TAR element of HIV and the viral protein Tat form a molecular switch regulating transcriptional efficiency in HIV. We show that fluorescence correlation spectroscopy at the single molecule level is a powerful method to study the association between a Tat‐derived peptide and TAR frag