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Fluorescence Analysis of the Labile Iron Pool of Mammalian Cells

✍ Scribed by Silvina Epsztejn; Or Kakhlon; Hava Glickstein; William Breuer; Z.Ioav Cabantchik

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
249 KB
Volume
248
Category
Article
ISSN
0003-2697

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✦ Synopsis

myeloid cells are in the range of 0.2-1.5 mM. The values varied commensurately with cell iron loads and iron The labile iron pool (LIP) of cells constitutes a cytosolic fraction of iron which is accessible to permeant chelator treatment. The method provides a simple, noninvasive tool for on-line monitoring of cytosolic chelators and contains the cells' metabolically and catalytically reactive iron. LIP is maintained by a bal-iron under normal and abnormal conditions of cell iron supply and for assessing the dynamics of intracel-anced movement of iron from extra-and intracellular sources. We describe here an approach for tracing LIP lular iron in living cells. α­§ 1997 Academic Press levels in living cells based on the fluorescent probe calcein (CA). This probe binds Fe(II) rapidly, stoichiometrically, and reversibly while forming fluorescencequenched CA-Fe complexes. Cells are loaded with CA

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## Abstract Iron regulatory proteins (IRPs), the cytosolic proteins involved in the maintenance of cellular iron homeostasis, bind to stem loop structures found in the mRNA of key proteins involved iron uptake, storage, and metabolism and regulate the expression of these proteins in response to cha