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A Review of Fluorescence Methods for Assessing Labile Iron in Cells and Biological Fluids

✍ Scribed by Breno P. Espósito; Silvina Epsztejn; William Breuer; Z.Ioav Cabantchik

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
355 KB
Volume
304
Category
Article
ISSN
0003-2697

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✦ Synopsis

A variety of biochemical, pharmacological, and toxicological properties have been attributed to labile forms of iron that are associated with cells or with biological fluids. Unlike the major fraction of bioiron which is protein bound, the labile bioiron is chelatable and therefore amenable for detection by metal-sensing devices that are coupled to chelation moieties. The present review deals with the detection of various labile forms of iron present in living cells and in fluids of biological interest, in health and disease. The main focus of the review is on the design and application of fluorescent probes as analytical tools for assessing labile iron and iron transport mechanisms and the efficiency of iron chelators in solution and in cellular systems.

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