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Flow microcalorimetric assay of antibiotics—III. Zinc bacitracin and its combinations with polymyxin B sulphate and neomycin sulphate on interaction with Micrococcus luteus

✍ Scribed by N.Joslin Kjeldsen; A.E. Beezer; R.J. Miles

Book ID
103911062
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
376 KB
Volume
7
Category
Article
ISSN
0731-7085

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✦ Synopsis

A flow microcalorimetric assay for zinc bacitracin has been developed which has better reproducibility (relative standard deviation less than 2%) and sensitivity (0.02 micrograms ml-1) than conventional microbiological assays, and requires an assay time of between 7.5-9 h. The assay is not suitable for zinc bacitracin determinations in the presence of equimolar concentrations of polymyxin B sulphate or neomycin sulphate, or of these antibiotics in the proportions in which they occur in the commercial preparation Trisep (ICI, Macclesfield, UK).

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