Flow Injection Fluoroimmunoassay for Human Transferrin Using a Protein a Immunoreactor
β Scribed by Fluoroimmunoassay, ; Ren, Xuezhen; Miller, J. N.
- Book ID
- 127332574
- Publisher
- Taylor and Francis Group
- Year
- 1994
- Tongue
- English
- Weight
- 287 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0003-2719
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We have used the bicinchoninic acid reagent developed by Pierce Chemical Co. to measure proteins in a simple flow injection analyzer. The sensitivity is comparable to that of the Lowry method and no pipetting of reagents is needed. Results are obtained in less than 1 min and samples may be run at a
within one assay series on one day and during several days. The intra-assay reproducibility was 1-3%, the inter-assay reproducibility 1-5% RSD. Preliminary experiments with serum samples have shown that there are no interferences and no sample pretreatment is necessary. The recovery from serum samp