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Flow cytometric analysis of group B streptococci phagocytosis and oxidative burst in human neutrophils and monocytes

✍ Scribed by Pamela S. McCloskey; Richard J. Salo


Book ID
114304590
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
321 KB
Volume
27
Category
Article
ISSN
0928-8244

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A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin-and antigendependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukoc