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Concurrent Measurement of Antigen- and Antibody-Dependent Oxidative Burst and Phagocytosis in Monocytes and Neutrophils

✍ Scribed by Carl-Fredrik Bassøe; Ingrid Smith; Steinar Sørnes; Alfred Halstensen; Anne Kristine Lehmann

Book ID
115639173
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
762 KB
Volume
21
Category
Article
ISSN
1046-2023

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✍ Anne Kristine Lehmann; Alfred Halstensen; Carl-Fredrik Bassøe 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB

A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin-and antigendependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukoc